GITR/NFκB-Luc/Jurkat
CBP74012
| I. Background | |
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糖皮質(zhì)激素誘導(dǎo)的腫瘤壞死因子受體相關(guān)蛋白（GITR，也稱為 TNFRSF18 或 CD357），屬于腫瘤壞死因子受體超家族（TNFRSF），它在多種免疫細(xì)胞上表達(dá)，包括 效應(yīng) T 細(xì)胞和調(diào)節(jié)性 T 細(xì)胞。GITR 在調(diào)節(jié)免疫系統(tǒng)中發(fā)揮重要作用，尤其是在 T 細(xì)胞的 活化和調(diào)節(jié)性 T 細(xì)胞（Treg）的功能中。 |
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| II. Description | |
GITR NFκB-Luc Jurkat 報告基因藥靶模型很好的模擬了體內(nèi) GITR 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。 Figure 1. GITR NFκB-Luc Jurkat 細(xì)胞模型原理圖
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: |
GITR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+400 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 2. Recombinant GITR/NFκB-Luc/Jurkat stably expressing GITR.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human TNFSF18 in GITR-NFκB-Luc Jurkat(C1). Dose Response of GITR NFκB-Luc Jurkat(C1) Stimulated by GITRL CHO (C1).
