ERBB Family Effector Reporter Cell
CBP74507
詢 價
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產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |
| ERBB 家族（又稱 HER家族）是一類在細(xì)胞生長、分化與存活中至關(guān)重要的受體酪氨酸激酶，其異常激活是驅(qū)動多種癌癥（如肺癌、乳腺癌、胃癌）的關(guān)鍵因素。該家族包含 四名成員：EGFR/HER1、HER2、HER3 和 HER4。它們各自的特點(diǎn)為：EGFR 擁有多種配 體；HER2 無已知直接配體但信號能力最強(qiáng)；HER3 激酶活性缺失，必須與其他成員協(xié)作； HER4 結(jié)構(gòu)最為復(fù)雜。其中，HER2 是最核心的信號放大器，而 HER3 是關(guān)鍵的信號伙伴， 兩者的異源二聚體是驅(qū)動腫瘤生長和耐藥的主要信號單位。 ERBB 家族通過配體誘導(dǎo)的二聚化機(jī)制啟動其核心信號通路。當(dāng)生長因子（配體）結(jié)合后，受體形成同源或異源二聚體（尤其是以 HER2 為中心的異源二聚體），引發(fā)胞內(nèi)酪氨酸激酶區(qū)的相互磷酸化。這些磷酸化位點(diǎn)隨后募集并激活下游兩條主要信號通路： RAS/MAPK 通路（調(diào)控基因表達(dá)和細(xì)胞增殖）與 PI3K/AKT 通路（調(diào)控細(xì)胞存活和代謝）。這條從膜受體到細(xì)胞核的強(qiáng)大信號傳導(dǎo)級聯(lián)，在正常情況下精密調(diào)控生理過程，一 旦因受體過表達(dá)或突變而失控，便會持續(xù)驅(qū)動腫瘤的生長、侵襲和生存，這也正是針對 EGFR 和 HER2 等靶點(diǎn)的藥物（如單克隆抗體、小分子抑制劑）能夠有效治療癌癥的根本原理。 |
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| II. Description | |
ERBB Family Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) ERBB 家族的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。 Figure 1. ERBB Family Effector Reporter Cell 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Expressed gene: | EGFR&HER2&HER3&HER4 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 +10%FBS + 100 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 2. WB of ERBB Family Effector Reporter Cell.  Figure 3. Recombinant ERBB Family Effector Reporter Cell stably expressing EGFR & ERBB2 & ERBB3.  Figure 4. Dose Response of Ligands in ERBB Family Effector Reporter Cell (C16).  Figure 5. Inhibition of Human EGF induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 6. Inhibition of Human EGF induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell( C16).  Figure 7. Inhibition of Human NRG1-β1 induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 8. Inhibition of Human NRG1-β1 induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell (C16).  Figure 9. Inhibition of Human NRG1-β2 induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 10. Inhibition of Human NRG1-β2 induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell (C16). |
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