hTF/MC-38
CBPG0107
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產(chǎn)品描述
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| I. Background | |
| 凝血因子 III (Coagulation Factor III)，又稱組織因子(Tissue Factor，TF)或 CD142，是外 在凝血途徑的主要調(diào)節(jié)因子。它缺乏內(nèi)在的蛋白水解活性，但可以以 Ca 2+依賴的方式結(jié)合 活化因子 VII（FVII）或活化因子 VIIa（FVIIa），并通過變構(gòu)增強(qiáng)該蛋白酶的酶活性，催 化因子 X (FX)活化為 FXa。由于其凝血特性，TF 在血栓形成或彌散性血管內(nèi)凝血等病理?xiàng)l 件下都具有重要作用。在動(dòng)脈粥樣硬化過程中，TF 大量表達(dá)，并且能夠促進(jìn)血栓形成。除 了發(fā)揮凝血作用外，TF 在炎癥、血管生成以及腫瘤生長和轉(zhuǎn)移中也具有一定作用。 | |
| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | hTF |
| Stability: | 32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hTF/MC-38 stably expressing TF. |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
C57BL/6J, 6weeks, female |
|
Implanted Position |
Subcutaneous (right lower flank region ) |
|
Injected Cell Numbers |
1×10^6/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hTF/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hTF/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
