NGF/hTRKA Effector Reporter Cell
CBP74174
| I. Background | |
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NGF 是神經(jīng)營養(yǎng)因子（NT）家族的成員，該家族還包括腦源性神經(jīng)營養(yǎng)因子 （BDNF）、神經(jīng)營養(yǎng)因子 3（NT-3）和神經(jīng)營養(yǎng)因子 4/5（NT-4/5）。神經(jīng)營養(yǎng)因子是發(fā) 育、維持和存活以及記憶形成和儲存的關(guān)鍵調(diào)節(jié)因子。NGF 在成年基底前腦膽堿能神經(jīng)元 （BFCN ）的刺激、存活和表型維持中起著關(guān)鍵作用。它在皮質(zhì)和海馬體中合成，并逆行 輸送到 BFCN 細胞體，觸發(fā)導(dǎo)致維持正常細胞功能和形態(tài)的過程。NGF 可以上調(diào)幾種膽堿 能標志物，即 ChAT 活性、基因表達和蛋白質(zhì)水平、乙酰膽堿合成和釋放以及囊泡乙酰膽 堿轉(zhuǎn)運蛋白（VAChT）的表達。 |
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| II. Description | |
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NGF/TRKA Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) NGF/TRKA 的 信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III.Introduction | |
| Expressed gene: | TRKA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10%DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 2. Recombinant NGF/hTRKA Effector Reporter Cell constitutively expressing TRKA.
Figure 3. Dose Response of Recombinant Human β-NGF Protein in NGF/TRKA Effector Reporter Cells(C21).
Figure 4. Inhibition of rhNGF-induced Reporter Activity by NGF Neutralization Ab in NGF/hTRKA Effector Reporter Cell(C21). Inhibition of rhNGF-induced Reporter Activity by TRKA Inhibitor in NGF/hTRKA Effector Reporter Cell(C21).
